I am a student of nutrition science at the university of Bonn (Germany) and I'm working on a study of immunophenotyping T-lymphocytes by three-color Flow cytometry. I want to get the percentages of the naive T-cells (CD45RA+) and memory T-cells (CD45RO+) in the blood samples. Therefore I used the antibodycombination of CD45RA-FITC, CD45RO-PE and CD3PerCP. I'm experiencing difficulties by setting the gates in the dot plots. I don't know where to set the gates for separating the two populations of cells (naive and memory T-cells).I don´t know what criteria I have to use to separete this two subpopulations. (I'm using the program WinMDI 2.7) I know there are some subpopulations between the naive and memory T-cells like double positive T-cells (CD45RA+CD45RO+) and this makes it very difficult to set the gates. I will be very obliged if you could tell me something about it or if you could tell me about related literature. Thank you very much in advanced. Monica Matas Nobis --------------------- Mónica Matas Nobis mailto:M.Matas@gmx.deReceived on Wed Aug 23 13:47:55 2000
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