Re:

Mary Stewart wrote-

>Does anyone have experience using LDS-751 for cell cycle analysis?  I'm
>looking for a cell permeant dye that will detect DNA in live GFP positive
>or negative cells.  We have a BD FACScan with a 488 argon laser, so I'm
>limited to what can be used with this laser.  Thanks for any help.

LDS751 is not DNA specific.  What you want is a new dye, DRAQ5, described
by Paul Smith <smithpj2@cf.ac.uk> et al in the August 1, 2000 issue of
Cytometry (40:280-291); this is the first dye of which I am aware that
gives reasonable DNA histograms from live cells with 488 nm excitation.

-Howard