(no subject)

>Hi Bill,
>I have used the method published by Chu in Cytometry 36, 333-339 (1999),
>bascically ice-cold 1% PFA incubated with cells on ice for 1h then the 70%
>ethanol step etc and finally PI. I have found that for some cell types you
>do get leakage, COS  cells were fine.
>
>
>Gary Warnes,
>Head of Flow Cytometry at Fluorescent Imaging
>MRC, Hammersmith Hospital,
>London, UK