Flowers-- I had a researcher come into the lab with a Eu-chelate as the fluorochrome. He came without literature and said it had an excitation peak around 345nm (I used the relatively weak 351nm line from an Enterprise) and a very narrow emission at 615nm (I used a 610/20 to collect). I was unable to see any kind of signal although his controls using a FITC-conjugate worked fine. I was a little suspicious when he said it was "quantumly inefficient" and he had never seen it tried on a flow cytometer (I'm betting it might need a little more time in the beam). Do any of the gurus out there have any wisdom to share. Al asaluk@scripps.eduReceived on Tue Jun 27 15:19:35 2000
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