We did a limited analysis and found ACD to be the best. However, at 48 hours the EDTA blood showed <12% variation from the freshly analyzed samples ( n=4). This isn't too bad, since we see up to 10% interassay variation. faisal rawas wrote: > Hi every body, > I would like to know if it is ok to use a 48 hr old peripheral blood that > was collected in EDTA for lymphocyte subsets using the MultiTEST reagents > (using the CD45 vs SSC gating strategy). Currently we are rejecting such > specimens on the bases that EDTA is not a great anti-coagulant to test for > lymphocyte subsets in blood that is older than 24 hr. any comments or > recommendations are welcome. > > Faisal Rawas > Senior-in -charge > Flow Cytometry Lab > King Faisal Hospital > Riyadh, KSA > > ______________________________________________________ > Get Your Private, Free Email at http://www.hotmail.com
This archive was generated by hypermail 2.1.8 : Fri Oct 07 2005 - 10:56:11 EST
