Hi, Flowers, I am still trying to find an easy solution for intracellular Ca concentration measurement. Can I assume that Ionomycin totally collapses the calcium gradient across the cell membrane and if the cells are suspended in a series of calibrated Ca buffers (like MP # C-3009, Calcium Calibration Buffer Kit #2) the intracellular concentration equals the buffer's? Thanks, Akos Akos Szilvasi Biogen Cambridge, MAReceived on Thu Mar 2 13:09:36 2000
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