Dear Flowpersons Can anyone inform me of how many mice (or mouse spleens) do I require for one run of EACH of the following assays? I have never done flow on mice and require this information for the Institute Animal Care and Use Committee. * CD4 * CD8 * NK activity * * Lymphocyte activation * * Phagocytosis (fluorescent beads) * * Proliferation of any cell type * * Reactive oxygen species * * PKC activity * * Phospholipase activity * * Intracellular pH * * Membrane potential Thank you, Steve Gaffin ************************************************************ ************************************************************ ** Stephen L. Gaffin, Ph.D. ** ** Research Physiologist ** ** U.S. Army Research Institute for Environmental Medicine ** ** Natick MA 01760-5007 ** ** Tel. 508-233-4867 Fax 508-233-4869 ** ** email stephen.gaffin@na.amedd.army.mil ** ************************************************************ ************************************************************Received on Wed Jan 19 13:12:26 2000
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