I am planning to study intracellular cytokine expression in lymphocytes following activation with PMA + ionomycin, but have concerns about reports of CD4 down regulation following activation. I would like to know if this is an 'all or none' phenomenon or if the CD4 remains expressed at a level sufficient to permit delineation from true CD4 negative cells. If you have experience here, I would appreciate your comments. Thanks. please reply to: kmaher@med.miami.eduReceived on Wed Jan 12 15:15:26 2000
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