From: Beth Rees (beth.rees@dchs.tas.gov.au)
Date: Wed Dec 08 1999 - 01:27:30 EST
>from what I hear one has to set a minimun number of events in that "rare" >population .. for our lab, it's 3000 cells, which is a lot of acquisition >if we're talking about something that's only a fraction of a percent >abundant! So bring a good book... I recommend Moby Dick :) >> >> Is it possible to get a comment on this issue from more of those people, who >> are dealing with rare events in hematology/oncology? >> What are the common procedures for detecting rare events in oncology >> utilizing flow cytometry? How is precision established and verified? > CD34 in blood or bone marrow often comprises a "rare event", eg. 0.05%, which can be analysed in a reproducible manner using a series of cumulative gates and backgating, with or without beads according to the method outlined in Sutherland et al, "The ISHAGE guidelines for CD34 determination by Flow Cytometry" J Hematotherapy 5:213-216 (1996), and Keeney et al, "Single Platform Flow Cytometric Absolute CD34 Cell Counts based on the ISHAGE Guidlines" Cytometry (Communications in Clinical Cytometry) 34:61-70 (1998). We usually count 50,000 events (total) in triplicate + a negative control, and get good reproducibility. Beth Rees Royal Hobart Hospital Hobart Tasmania > >===== >`---------------------------------------------` >| Maciej S. Simm | 525 E 68th Street | >| Research Technician | Room N-805 | >| Cornell Medical Center | Tel. 212.746.3428 | >`---------------------------------------------` >__________________________________________________ >Do You Yahoo!? >Thousands of Stores. Millions of Products. All in one place. >Yahoo! Shopping: http://shopping.yahoo.com
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