From: Michael Ormerod (Michael_Ormerod@compuserve.com)
Date: Sun Nov 28 1999 - 15:30:38 EST
Ihave not worked on this system myself but I am told that the He-Ne laser will excite the Cy5 on the PE-Cy5 conjugate directly, which accounts for the need to apply so much compensation. Looking at the excitation/emission spectra, this is what would be expected. I think that it might be best to use PerCP as the third dye. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: +44 (0)1737 241726 PLEASE NOTE NEW FAX & MOBILE NUMBERS FAX: +44 (0)1737 226736 Mobile telephone: 07802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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