Re: synchronizing T cells.

From: Andrew D. Wells, Ph.D. (adwells@mail.MED.UPENN.EDU)
Date: Wed Nov 17 1999 - 15:12:16 EST

Next message: Aaron Rae: "Re: Power Mac G4's and Cellquest"
Previous message: Van Bockstaele, Dirk: "high speed sorters: survey of customers satisfaction"
In reply to: Mauricio Caetano: "synchronizing T cells."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

>Hi people!!!!
>
>Does anybody knows any protocol to sychronize T cells in order to measure
>the cell cycle time?


Mimosine (250 microM) will arrest T cells in G1.  There are certainly a
myriad of drugs that one could use, but I've found mimosine to have certain
advantages; cells can remain arrested for several days without appreciabe
loss in viability, and the arrest is reversible.

AW


Andrew D. Wells, Ph.D.
University of Pennsylvania
Department of Medicine
904 Stellar-Chance Laboratories
422 Curie Boulevard
Philadelphia, PA  19104
(215) 898-1951
(215) 573-2880 (FAX)
adwells@mail.med.upenn.edu

Next message: Aaron Rae: "Re: Power Mac G4's and Cellquest"
Previous message: Van Bockstaele, Dirk: "high speed sorters: survey of customers satisfaction"
In reply to: Mauricio Caetano: "synchronizing T cells."
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:37:29 EST