From: Jonathan Porter (J.D.Porter@exeter.ac.uk)
Date: Thu Oct 07 1999 - 02:52:16 EST
Andrew, > I'm considering IMS as a way of seperating filamentous bacteria > (Microthrix parvicella) from activated sludge samples. Has anyone > recently done anything similar, and also any comments on the various > brands available. I'm considering Dynal and Advanced Magnetics > antiRabbit IGg products, but have heard Dynal beads give low recovery > rates. I've done similar things, though not with filamentous bacteria. I'm puzzled as to why Dynal beads are reputed to give low recovery rates. The limiting factor as far as I could tell was the hapten and the labelling step. Recovering the beads was rarely a problem, even from blended soil samples. Linking the hapten onto the beads whilst maintaining reactivity was more of a problem, but it was straightforward to improve this by reacting biotinylated hapten with the cells and then adding streptavidin coated beads. Jonathan _________________ Jonathan Porter School of Biological Sciences, University of Exeter, UK.
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