From: Andrew D. Wells, Ph.D. (adwells@mail.MED.UPENN.EDU)
Date: Tue Sep 14 1999 - 17:04:53 EST
>Dear all: > >we are searching for a dead-cell exclusion die to be detected in FL-4 (we >have a 2 laser FACSCalibur). Has any of you had any experience with TO-PRO-3 >(sold by Molecular Probes), so to either suggest or warmly disencourage its >use? Alternatively, did you come across to some other possible candidate? >Thanks. Alessandra, We have used TOPRO-3 as an FL-4 vital dye with much success (see J. Clin. Invest. 100:3173) at a final conc. of about 5 nM. AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 904 Stellar-Chance Laboratories 422 Curie Boulevard Philadelphia, PA 19104 (215) 898-1951 (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
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