From: Gregor Rothe (gregor.rothe@klinik.uni-regensburg.de)
Date: Wed Aug 04 1999 - 11:04:24 EST
Hello to all, The analysis of H-Y antigen expression on leukocytes by flow cytometry already a long time ago has been published as a method to discriminate neutrophils from male and female subjects (Kent M. et al. Hum Genet 1990; 85:127-9). Although this method might be highly relevant for the analysis of chimaerism in sex-mismatched bone marrow transplantation, I have never seen any further publications on this methodology. The only exception is a recent report that H-Y expression does not reliably discriminate male from female sperm in human. Can anyone report on any experience with this or an alternate technology? Gregor Rothe *********************************************************** Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg Franz-Josef-Strauss-Allee 11 D-93053 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 E-mail: gregor.rothe@klinik.uni-regensburg.de ***********************************************************
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