BrdU Incorporation

From: Bill Throndset (bthrondset@rigel.com)
Date: Tue Jul 27 1999 - 18:25:27 EST

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Dear Group;

I am looking for BrdU incoporation protocols other than Becton Dickinson's
protocol. I have tried twice with B.D.'s protocol but it didn't work for my
Hela cells. Because my Hela cells express GFP, I can't use direct labeling.
I used Pharmigen's anti-BrdU, Biotin-mIgG1 as secodary antibody, and SA-APC
or SA-perCP.

Thanks for any suggestions.


Bill Throndset
Flow Cytometry Consultant
Rigel, Inc.
240 East Grand Avenue
South San Francisco, CA 94080
650-624-1231
650-624-1101 (fax)
bthrondset@rigel.com

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