Re: CellQuest Question

From: Eric Van Buren (aa9080@wayne.edu)
Date: Fri Jun 18 1999 - 12:41:35 EST

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Chuck Radford wrote:
>Does anyone out there know how to calculate the ratio
>of fluorescence to FALS of each cell and collect it
>as a histogram using CellQuest?  I know that Cicero
>software is capable of doing that, but I'm not sure
>if CellQuest can do that.

This is not possible with CellQuest. However, it should be possible with
each of these Macintosh programs: FlowJo <http://www.treestar.com/> or FCS
Assistant <http://facsmac.med.cam.ac.uk/fcsa.html>. CALC4 for VAX/VMS by
Bob Murphy was a nice program for this kind of application, but it only
works with LACEL and FCS1.0 files.

Eric

/\/\/\_ Eric Van Buren, aa9080@wayne.edu
\ \ \   Karmanos Cancer Institute and Immunology & Microbiology
 \_^_/  Wayne State University, Detroit, Michigan, USA

Next message: Turner, Julie: "RE: CD14 and trypsin (and other receptors, too)"
Previous message: Mario Roederer: "The "perfect" software ... also does ratios... and calibrated parameters..."
In reply to: Chuck Radford: "CellQuest Question"
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