FACS on gram+ bacteria

From: Mario Roederer (Roederer@Stanford.edu)
Date: Fri Jun 04 1999 - 18:19:09 EST

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We have a group that is trying to detect antibody staining of antigens on
the surface of gram-positive bacteria.  Does anyone have experience with
this?  (Or, more generally, analysis of gram+, or of surface antibody
staining on gram-negatives).  We'd be interested in seeing some typical
methods (what colors do you use, do you use second step reagents) and to
see some typical profiles of both scatter and fluorescence.

Thanks for any information!

mr

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