Ratiometric calcium measurement

From: Beth Whalen (BWhalen@prl.pulmonary.ubc.ca)
Date: Tue May 04 1999 - 15:54:00 EST

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Hello everyone,

I have just started a new project measuring the change in intracellular calcium 
in neutrophils using flow cytometry.  No one in our lab has done this before so I 
just started using fluo-3 because we had some.  In my readings I am lead to 
believe that a ratiometric approach using fluo-3/fura red is a better way to go.  I 
have done a literature search and have only found one reference by Novak and 
Rabinovitch in 1994 describing the method.

I would like to know:

	1.  Which method people are currently using for a bench top analyzer 
	with a 488nm argon laser?  

	2. Is anyone really using the fluo-3/fura red method?

	3.  Is this method better than just using fluo-3 alone?

Your input will be greatly appreciated.

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