From: Kathleen Schell (kschell@facstaff.wisc.edu)
Date: Fri Apr 09 1999 - 17:34:17 EST
Does anyone know if a live-dead excluder like Propidium iodide or 7-AAD could contaminate a sort of live cells enough to interfere with mRNA measurements or PCR? Even after washing? We have an investigator who insists on not using any dyes except monoclonal antibodies during sorts of fresh tissue. We have had difficulty excluding dead and dying cells based on scatter and autofluorescence and would realllllly like to include a dye to help us. Any suggestions or comments? Thanks. Kathy -------------------------------------------------------------- Kathy Schell Supervisor, UWCCC Flow Cytometry Facility 600 Highland Ave. K4/535 Madison, WI 53792 Voice: 608-263-0313 e-mail: kschell@facstaff.wisc.edu
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