From: Rohan and Shanthi Ameratunga (immunology@xtra.co.nz)
Date: Thu Apr 01 1999 - 17:39:01 EST
Antony, No problem, I'll post the replies next week. Thank to all who responded Rohan Ameratunga Auckland Hospital New Zealand Antony Bakke wrote: > I would also be interested in any responses to this question, so please post to the list. > > Thank you, > > Antony Bakke, PhD > Oregon Health Sci Univ > Dept of Pathology > bakkea@ohsu.edu > > >>> Rohan and Shanthi Ameratunga <immunology@xtra.co.nz> 03/30 8:20 PM >>> > > Dear Colleagues, > > I would be grateful for advice on CTLA4 staining. We are attempting to > stain cell surface CTLA4 as marker of solid organ rejection in > peripheral blood lymphocytes. None of liver or kidney transplant > samples have been positive using the Coulter immunotech antibody. We > are not sure if this is a technical problem or whether the antigen is > not expressed in the periphery. > > 1. What in vitro stimulation methods should be used for positive > controls? How long should cells be stimulated before we detect CTLA4? > > 2. How useful is intracellular staining for this antigen? > > Thank you in advance > > Best regards > > Rohan Ameratunga > Auckland Hospital > NEW ZEALAND
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