Re: Sorting C. elegans embryos

From: N.W.BLACKHALL (Nigel.Blackhall@nottingham.ac.uk)
Date: Fri Mar 12 1999 - 04:12:49 EST

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Dear all,

Wayne Green wondeed why his recovery was low when sorting C. 
elegans.

Wayne was using a Vantage with a 100 micron tip (no Macro Sort).
The embryos are 50x30 microns size.

I feel that the size of the embryos is the problem.  As a rule of 
thumb I believe that the tip diameter should be 3 or 4 times the 
size of the largest particle being sorted to achieve efficient 
recovery, i.e. 150-200 microns in this case.

Yours sincerely,
Nigel Blackhall,
Experimental Officer, Plant Science Division,
School of Biological Sciences, University of Nottingham,
University Park, Nottingham NG7 2RD, UK
Tel +44 115 9515151 ext 18501
Fax +44 115 9513298

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