Re: monocyte seperation

From: smonard@adarc.org
Date: Thu Feb 11 1999 - 16:17:40 EST

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Hi

Something key in separating monocytes by FACS is is include some EDTA in
your buffer the monoctes are suspended in, 5mM should do. Without EDTA
monocytes disappear from the suspension, sticking to tubing, each other or
something. You should be able to get  1 million monocytes from 20 ml blood
from most individuals. So just ficol your cells, stain with CD14 (purity
isn't that bad if you just use scatter) and suspend in PBS with 5mM EDTA
and sort

Simon Monard
Aaron Diamond Center
NYC

• Next message: Mary Paniagua: "Position available: Chicago area Flow Core Lab"
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• Maybe in reply to: Mats Alheim: "monocyte seperation"
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