From: Michael Ormerod (Michael_Ormerod@compuserve.com)
Date: Wed Jan 13 1999 - 06:31:23 EST
I cannot be definite without seeing the data, but it sounds that you are looking at 'doublets'; that is, two cells in G1 stuck together. They will have the same DNA content(and hence integrated area signal) as a cell in G2/M but will have a greater width. The purpose of meaasuring area and width of a DNA signal is to recognise these cells and to exclude them from the analysis. I assume that you are using a BD machine. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: voice & FAX: (0)1737 241726 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:36:52 EST
