size of genome by FACS

From: Marina Polonskaia (mpolonskaia@hms.harvard.edu)
Date: Mon Jan 04 1999 - 17:56:25 EST

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Dear ALL,

I have a user who wants to measure the genome size of an animal called an
acorn worm. They (and I) never did anything like that, I always worked with
mammalian cells. Does anyone can suggest what kind of control we should use
and what to take for analysis - sperm or nucleus? Is any special
preparation required?
Any information would be useful.

Thank you very much.
Marina

Dr. Marina G. Polonskaia
Research Associate
Harvard Medical School
Flow Cytometry Facility
tel: (617)432-3647
pager:(617)465-8982
e-mail: mpolonskaia@hms.harvard.edu

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