Re: Fixation of GFP containing cells

From: Saverio Alberti (alberti@cmns.mnegri.it)
Date: Fri Dec 11 1998 - 08:24:54 EST

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4% paraformaldehyde in PBS, 10 min on ice, works beautifully.
Acetone/methanol and other precipitating fixatives also work well, but
fluorescence decays over a few days/weeks, possibly because of GFP
proteolysis.

Saverio Alberti
Head, Unit of Experimental Oncology
Department of Cell Biology and Oncology
Consorzio Mario Negri Sud
66030 Santa Maria Imbaro (Chieti), Italy
Phone: (39) 872-570.293
FAX: (39) 872-578.240
E-mail: alberti@cmns.mnegri.it


On Wed, 9 Dec 1998, Lucy Brown wrote:

> 
> 
>      Hello all,
>      
>      I have a user who is interested in fixing her GFP expressing cells 
>      before flow cytometry analysis.  Has anyone used any fixation methods 
>      that preserved the GFP expression?
>      
>      Thanks for any advice,
>      Lucy Brown
>      Beckman Research Institute/City of Hope
>      626-359-8111 X3306
> 
> 
>

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