From: biocytex@biocytex.com
Date: Fri Dec 11 1998 - 09:28:13 EST
Hi Dalia, A lymphocyte histogram always contains 2 populations of CD8+ cells.The CD8 high (bright) are exclusively T cells (CD3+) and the CD8 intermediate include both NK (CD3neg., generally representing the majority) and T cells (CD3+, TCR gamma-delta+). The CD8high express both alpha and beta chains of CD8 as heterodimers and bind high levels of most CD8 MAbs (120-160,000 molecules/cell, see Bikoué et al. , Cytometry (1996),26:137-147). The CD8 interm. express only one chain as homodimers and bind lesser amounts of CD8 MAbs. This make them appear dimmer. We do see them in normal samples when doing CD8/CD3 dual staining. However the proportion of CD8 inter. T PBL is generally so low in healthy people that they often may not be taken into account. Good success with your PhD! Philippe Poncelet Director R&D BioCytex -----Message d'origine----- De : Dalia and Natasha <d.khalil@mailbox.uq.edu.au> À : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> Date : jeudi 10 décembre 1998 18:59 Objet : CD3+ CD8+ intermediates > >Greetings! > > Our research group has noticed that in few patient's PBMC, there has >been two populations of CD3+ CD8+ cells. One is of a high fluorescence and >the other intermediate. We regularly observe the high fluorescent CD3+8+ >population, but only a few have a definate second, intermediate fluorescent >CD3+8+ population. Has anyone also observed this second population, and do >you know what type of cells these are? > >Thankyou! >Dalia (PhD student) > >************************************************************************** >University of Queensland >Mater Hospital >1st Floor, Administration Centre >South Brisbane Queensland 4101 Australia >Telephone: (07) 3840 8518 >International: +61 7 3840 8518 >Facsimile: (07) 3840 1548 >************************************************************************** > >
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