From: Darren Hickerson (dhickerson@brody.med.ecu.edu)
Date: Tue Dec 01 1998 - 13:31:03 EST
A graduate student is interested in labeling thiol groups in platelets by flow. She would like to differentiate between intracellular and extracellular labeling. A) Has anyone done this in any cell type (neither of us can find references)? B) Is anyone familiar with using any of the following compounds by flow? fluorescein iodoacetamide, fluorescein maleimide, Oregon Green 488 iodoacetamide, or tetramethyl rhodamine iodoacetamide. C) As an alternate procedure, has anyone attempted staining intracellular GPIIbIIIa to differentiate it from surface GPIIbIIIa using MoAbs by flow? If yes to any of these, please send a quick note with information regarding specific products, hints as to an appropriate protocol, or what references may be available that we could look up. Please e-mail directly to me if possible. Thank you for your time. Darren Hickerson Core Flow Cytometry Facility Brody 4W37 Department of Microbiology & Immunology East Carolina University School of Medicine Greenville, NC 27858 (252) 816-2799 dhickerson@brody.med.ecu.edu dhicker@aol.com
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