Subtraction of Histograms

From: Christopher S Boyce (csboyce@beckman.com)
Date: Tue Nov 24 1998 - 18:08:48 EST

• Next message: Mihaela Roxana Cimpan: "flow statistics - novice's questions"
• Previous message: Khalid Al-Hussein: "Position open"
• Next in thread: Adrian Smith: "Re: Subtraction of Histograms"
• Reply: Adrian Smith: "Re: Subtraction of Histograms"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

Hello FLOWers,

Is there anyone who has used the subtraction histogram function on
CellQuest?  How useful is this
quasi-histogram?  Is there an appropriate time when finding the difference
between two histograms this way
is valid?   And then what about overlaying these histograms with others?
Any comments?  It seems reasonable
to use this function when comparing {isotype and/or unstained samples-test
sample pairs} with other
{isotype and/or unstained- test samples}.  Would it  provide you with a sum
histogram, that when compared with other sets, compensate for the  slight
differences in autofluorescence between sets?  Or is the subtraction
histogram
a fancy software component of little use?


Best Regards,

Christopher Boyce

• Next message: Mihaela Roxana Cimpan: "flow statistics - novice's questions"
• Previous message: Khalid Al-Hussein: "Position open"
• Next in thread: Adrian Smith: "Re: Subtraction of Histograms"
• Reply: Adrian Smith: "Re: Subtraction of Histograms"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]

This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:35:27 EST