From: Steve G. Hilliard (steve@habanero.cb.uga.edu)
Date: Fri Nov 20 1998 - 15:41:59 EST
Brian Binder and I were just working out the volume of sort droplets
when this came note came in. Aside from the empirical (sort and measure),
we looked at it this way:
Calculate the flow rate in mls/s, and multiply by the duration of
droplet formation.
Assume stream velocity (v) = 10m/s
Orifice diameter (d) = 100um
Droplet drive frequency = 25kHz
Duration of the drop (the amount of time represented by each drop) is
equal to the reciprocal of drive frequency in Hz, so a drive frequency
of 25000 = 4x10^-5 sec. To calculate the flow rate, think of it as a
column of fluid with diameter d and height = v (10m/s) (I know it sounds
screwy, but it works). The volume of a cylinder = pi (r)^2h. Convert
all the distances to cm to make it easy and you get a flow rate of 0.078
ml/s. Multiply that times the duration of drop formation and you get a
droplet volume of 0.003ml.
Someone emailed me this answer months ago but I lost it.
Steve
-------------------------------------------------------------
Steve G. Hilliard flowman@uga.edu
University of Georgia Cell Analysis Facility
http://floweb.cb.uga.edu/Floweb/
-------------------------------------------------------------
On Wed, 18 Nov 1998 jeff_carrell@hgsi.com wrote:
>
>
> Hello all,
>
> How can I determine the volume of a sorted cell and it's surrounding drop? I am
> using a Vantage to sort into Terasaki plates, and the investigator would like to
> exactly calculate concentration of cytokines in the collection medium. Is this
> possible?
>
> Thanks,
> Jeff Carrell
> Human Genome Sciences
>
>
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