From: Arnold Pizzey (a.pizzey@ucl.ac.uk)
Date: Mon Nov 23 1998 - 04:22:20 EST
>Hi all!
>
>Could anyone please enlighten me on why methanol is used instead of ethanol
>by some investigators for fixation of cells prior to propidium iodide
>staining? In many cases, I've seen methanol used after the investigators
>stain with a cell surface marker (e.g. CD20-FITC). Does the methanol
>preserve the fluorescence of the antibody better?
>
>Rizwan Haq
>haqr@oci.utoronto.ca
Just a quick remark;
I find that fixation with 70% methanol gives lower c.vs when staining with
Propidium Iodide or ToPro, I have looked at staining with CD10,CD11B,Oct2.1
under these conditions and see no degredation in signal
Best wishes
Arnold.
--------------------------------
Arnold Richard Pizzey
Department of Haematology
98 Chenies Mews
London WC1E 6HX
a.pizzey@ucl.ac.uk
(044) 0171 209 6234
fax: 0171 209 6222
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