From: Marcus Reckermann (recker@ftz-west.uni-kiel.de)
Date: Fri Nov 20 1998 - 07:26:11 EST
Brian, not being a DNA gugu, I would think that it is a wise idea to gate on your cell population, and have the model work on that exlusively. Cheers, marcus > A question to all of the DNA guru's out there. With the emergence of all > of the DNA modeling programs in the last few years that model not only > the cell cycle but also aggregates and debris, is it better to model all > of the events or to gate like in the old days on width vs. area and light > scatter before modeling? I have noticed that often times by gating the > data the CV can be lowered and the G2/G1 ratio is closer to 2.00, but > sometimes the opposite is true. Should you try both and choose the one > that looks better for that experiment or is it better to stay consistent > (which is what I would choose to do). Any thoughts appreciated. > > Brian Newsom > Center for Cell and Gene Therapy > Baylor College of Medicine > > __________________________________________________ Dr. Marcus Reckermann Research- and Technology Centre Westcoast (FTZ) Hafentoern D-25761 Buesum Germany Tel: +49-4834-604-204 or -261 Fax: +49-4834-604-299 E-Mail: recker@ftz-west.uni-kiel.de http://www.uni-kiel.de:8080/ftzwest/ Flow Cytometry Homepage: http://www.uni-kiel.de:8080/ftzwest/ag1/flow/flow-home-e.htm
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