From: Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Date: Tue Nov 17 1998 - 12:59:08 EST
Also- I haven't seen very bright CD38 in pre plasma cell B-cells. At least not as bright as we are talking about in plasma cells. Maryalice > >Correct me if I am wrong, but these plasma cells will be CD38 positive only. >No other B or T or other marker will be positive for these cells. > >jim > > -----Original Message----- > From: jsegal@adarc.org [SMTP:jsegal@adarc.org] > Sent: Friday, November 13, 1998 10:21 AM > To: Cytometry Mailing List > Subject: CD138 summary > > > Hi, > > Thanks to all who responded about CD138. The consensus seems to be >that > CD138 is indeed a decent plasma cell marker. Some suggested using >CD38 > (not to be confused with CD138) and looking for super-bright >expressers, > but in my experience all B cells stain very bright for CD38, so this >left > me a little confused. > > CD138 providers: > > Miltenyi Biotech - PE conjugate (will give free sample): >1-800-367-6227 > Research Diagnostics - FITC/PE > Serotec - FITC/PE > Caltag - PE > DAKO makes an "anti-plasma cell reagent" (intracellular) > > > Thanks again, > > Jeremy Segal > Flow Cytometry Laboratory > Aaron Diamond AIDS Research Center > NY, NY > > Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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