From: Kushman, Mary Elizabeth (MEK0705@mail.ecu.edu)
Date: Wed Nov 11 1998 - 15:00:54 EST
I have recently been investigating the effects of oligomycin (a mixture of A, B, and C) on the mitochondrial membrane potential in carcinoma cells. The delta psi indicator is Rh-123 dissolved in double-distilled water. However, I have had trouble dissolving the oligomycin. I tried absolute ethanol, that didn't work, and finally tried DMSO. That worked, but injection into my cell sample for flow resulted in precipation of the oligomycin in the cell suspension. Any of you out there that work with oligomycin have any info on how to properly dissolve it? (Yes, I have looked in the Merck Index and found that Oligomycin A is soluble in water, ethanol, benzene, acetone, etc....it's in the reference). Initial experiments show no change in delta psi when treated with the oligomycin, but since the oligomycin has precipitated out, no wonder no changes are occurring. Any of you that can offer any insight into this problem will have your responses greatly appreciated by me! Thank you in advance for any help. Best Regards, Mary Kushman East Carolina University Greenville, NC
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