DMSO as a solvent

From: Kushman, Mary Elizabeth (MEK0705@mail.ecu.edu)
Date: Tue Nov 03 1998 - 11:16:20 EST

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Hi all in FlowLand--

I have been using rhodamine-123 as an indicator of the mitochondrial
membrane potential in human fibroblasts for quite some time with good
results.  Currently, my stock is dissolved in double distilled water.
However, lately I have had to run some experiments to test the effects of
DMSO on the rhodamine fluorescence in these cells, and I am finding that the
DMSO is increasing the Rh-123 fluorescence by 20-25%.  Is this a normal
phenomenon?  I am posing this question because some other dyes, namely
Fluo-4 and Rhod-2, are dissolved in DMSO and I am concerned that this
solvent is skewing my results (which is important because I have my master's
thesis on the line!).

Speaking of fluo-4, have any of you had experience with it?  I am obtaining
very broad fluorescence peaks when I use it.  Is that normal?

Thanks in advance for any information!!

Mary Kushman
East Carolina University
MS Molecular Biology Program

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