From: Steve Perfetto (sperfetto@pasteur.hjf.org)
Date: Mon Nov 02 1998 - 08:25:34 EST
Zip,
As I recall the best autofluorescent blocker we found which worked well with the
FISH exps. we were doing using DIG_FITC was 0.4% evans blue. Try it and let me
know how it worked.
Stephen P. Perfetto, MS.,MT. (ASCP)
Walter Reed Army Institute of Research
Department of Molecular Diagnostics and Pathogenesis
1600 East Gude Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: Baboon auto-fluorescence ...
From: huw.gray@biotransplant.com at Internet_Gateway
Date: 10/28/98 3:31 PM
From: Huw Gray@BIOTRANSPLANT on 10/28/98 03:31 PM
To: cyto-inbox
cc:
Subject: Baboon auto-fluorescence ...
Greetings,
We have a problem with measuring E-GFP fluorescence in
transfected baboon cells. These exhibit marked auto-fluorescence in
the green (FITC) band when excited with 488nm. light, which obscures
the GFP signal. Now, I vaguely remember hearing somewhere (?) that if
one excites at an alternative wavelength (I have an argon, mixed gas &
HeNe lasers available, lucky me!) then this auto-fluorescence can be
reduced & the GFP measured still. Is this the case & if so, what might
be the appropriate excitation (& emission?) wavelengths ... ???
Suitably appreciative,
"Zip".
>>>--->
=============================================================
Huw S. ("Zip") Kruger Gray, Ph.D. | BioTransplant Inc.,
Scientist: Flow Cytometry. | Building 75,
------------------------------------------------------------|
Third Avenue,
'Phone: (617)-241-5200, extension: 254/256. | Charlestown Navy
Yard,
Fax: " " -8780. | Charlestown,
E-mail: Zip@BioTransplant.com | MA, 02129, U.S.A.
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