Macrosort on the FACSVantage

From: HANDLEY@sorter.dfci.harvard.edu
Date: Thu Oct 29 1998 - 13:33:58 EST

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Hi Everyone,

I am trying to set up our FACSVantage to sort 90micron spheres.  I want
to use the 400micron nozzle, but getting side streams is (for me) impossible.
I would appreciate suggestions from anyone who has experience with large
particles.  Are there any "tricks" that you use that aren't in the manual?
Usually, the largest cells we work with are HELA cells, so are there 
other helpful hints to keep in mind when sorting large particles?  

ANY suggestions are welcome!
Thanks,
Maris

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Maris A. Handley                    Phone: (617)632-3139 (voice mail)
Dana-Farber Cancer Institute               (617)632-3179 (laboratory)
J319A, 44 Binney St.                
Boston, MA  02115                   E-mail: handley@sorter.dfci.harvard.edu
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