Re[2]: Reporting Median Values

From: Ben Verwer (Ben_Verwer@BDIS.Com)
Date: Wed Oct 28 1998 - 13:10:48 EST


Ray,

The short answer to: "Do those sorters have 256 channels per decade, or do they 
have four decades?", is: 256 channels per decade.

Here's what I've been able to find:

The front panel display on the FACStar (or FACStar+) had been designed at first 
for 256 channels, and had only 3 digits to display the sort windows in. When 
the decision was made to use 10 bit ADC's, rather than delaying release, the 
team decided to collapse everything greater than 1000 into channel 1000. 1000 
is the value send to the computer. If the sort window is set at 999, the sort 
window will expand to 1023 (or its analog equivalent as the rectangular sort 
windows are done in analog). 

In the Vantage SE the analog rectangular sort windows are no more and the values
now range from 0-1023, so the answer is: 256 channels per decade AND 4 decades.


As for the conversion between channels and linear values, CellQuest uses the 
formula: 10^(4*channel/1024), so the value 10000 is never reached. To add 1 to 
the channel number to make it reach 10000, at the expense of reaching the value 
1 at the bottom would be equally valid, but since we currently do it the other 
way, I don't see the big advantage of changing.


Ben


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