From: Jo Beaver (beaver@science.canberra.edu.au)
Date: Sun Oct 25 1998 - 17:08:26 EST
I have used PI staining of ethanol fixed cells extensively after incubation conditions I know will produce either apoptosis or necrosis. I have verified the outcome with EM amongst other tests. A cell population that contains a significant proportion of necrotic cells usually shows up with a very tight G0/G1 peak. This peak often has a cv tighter than the negative control cells. This is a bit tricky as it makes the detection of "saving" of cells from apoptosis difficult to prove with this flow method. I have found several treatments that seemed to "save" cells from apoptosis (as recognised by the absence of the sub G0/G1 population) in fact led to the cell's death by necrosis. Jo Dr Jo Beaver Lecturer in Biochemistry School of Human & Biomedical Sciences ph: 61 6 (02) 6201 2533 Faculty of Applied Science University of Canberra ACT 2616 Australia fax: 61 6 (02) 6201 5727 email: beaver@science.canberra.edu.au URL: http://biomed.canberra.edu.au/ and http://gadi.canberra.edu.au
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