From: Calman Prussin (CPRUSSIN@atlas.niaid.nih.gov)
Date: Fri Oct 23 1998 - 12:53:52 EST
Mario is correct, I transposed an alpha with a beta. Should read: There are antibodies that recognize CD8alpha (virtually all of those out there) and mAbs that recognize CD8beta (the Immunotech clone 5F2, PHGM has a clone which recognizes mouse CD8b). IEL are CD8aa, whereas most circulating CD8 cells are CD8ab. Thus you will need to use both mAbs simultaneously to discriminate the IELs which will be CD8a+, CD8b-. Mainstream cells will be CD8a+, CD8b+. > ---------- > From: Mario Roederer > Sent: Friday, October 23, 1998 13:31 > To: Calman Prussin > Subject: Re: anti CD8alpha, beta > > > There are antibodies that recognize CD8beta (virtually > > all of those out there) and mAbs that recognize CD8alpha > > Are you sure? I find that most anti-CD8 antibodies recognize alpha > (although > CD8aa is lower affinity that CD8ab); only the rare antibody binds only to > CD8b. > To distinguish iIEL, we must use standard CD8 together with anti-CD8b, and > look > at CD8+CD8b-. > > mr >
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