CD45RA

From: Miller, Judith E. (judy_miller@merck.com)
Date: Thu Oct 15 1998 - 08:12:12 EST

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> We are trying to stain B cells from rat spleen using anti-CD45RA
> (Pharmingen, PE conjugate). We do not fix the cells in formaldehyde since
> it is known to alter CD45RA staining.  In some experiments, we see clean
> separation between stained and unstained cells.  However, in other
> experiments there is a small population of cells (approx. 5-15%) that is
> between the two peaks or near the positive peak.  The PBL from the same
> animal has a clean positive peak for CD45RA.  We are not sure if this is a
> real finding, a problem with our sample preparation method or the
> antibody.
> 
> We would appreciate any help on this matter.
> 
> Regards,
> 
> Tom Kawabata and Judy Miller
> Safety Assessment
> Merck Research Laboratories
> West Point, PA
> 

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