From: Derek Davies (daviesd2@icrf.icnet.uk)
Date: Mon Oct 05 1998 - 04:14:10 EST
Hi Jeffrey, For all my UV lasers I use beads from Polysciences (Fluoresbrite YG microspheres) which come in a variety of sizes from 0.05u up to about 100u. They are excited by a whole range of wavelengths from UV upwards (They are even OK for He-Ne 635nm excitation). For all UV work though I also like to use Hoechst stained HL60 cells for fine tweaking of the alignment as you should be able to get a very sharp G1 peak. hope this helps! Derek On Fri, 2 Oct 1998, Jeffrey Shain Haug wrote: > Fellow Cytometrist, > I have an Omnichome air cooled UV laser that I have never been able > to align well (low CVs for DNA work). I think, part of my problem is that > the Coulter beads I use aren't excited well by this laser. Does any one > know of some beads that have a broad excitation pattern when hit with a UV > beam? **************************************************************************** * Derek Davies Voice: (44) 0171 269 3394 * * FACS Laboratory, FAX: (44) 0171 269 3100 * * Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk * * London, UK * * * * Web Page: http://www.icnet.uk/axp/facs/davies/index.html * ****************************************************************************
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:35:24 EST
