Re: post sort mortality

From: N.W. BLACKHALL (Nigel.Blackhall@Nottingham.ac.uk)
Date: Fri Oct 02 1998 - 03:44:45 EST

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Dear all,

Claude Cantin wrote

> As it turns out, I now have a very serious viability problem with both
> primary and secondary cells sorted since our FACStar plus was 
> upgraded with the Turbo Sort Option.  Cell lines have suffered since 
> installation .....

> Standard TSO sorting conditions:  30 PSI sheath pressure; 70 micron nozzle;


Is it possible that your cells are mechanicaly fragile and object to 
being 'blasted' through a narrow orifice at 30 psi.

Just a thought!!

Best wishes
Nigel Blackhall,
Experimental Officer, Plant Science Division,
School of Biology, University of Nottingham,
University Park, Nottingham NG7 2RD, UK
Tel +44 115 9515151 ext 18501
Fax +44 115 9513240

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