Hemacytometer question

From: JCS (JSU800@prodigy.net)
Date: Mon Sep 28 1998 - 18:25:19 EST


Hi,

I am a newbie to this newsgroup, so don't mean to intrude. I have a question
about hemacytometer: I am thinking of getting one to count the number of
cells cultured in my experiment (degradation study of biomaterials). Now,
how many cells does one usually count to achieve a high precision rate? I am
also afraid that in order to achieve an uniform distribution of cells before
counted, a shake or two is required--->and wouldn't this tear up some cells
due to shear forces, etc?

All suggestions and comments will be appreciated. Thanks!

Joe


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