From: Keith Bahjat (kbahjat@ufl.edu)
Date: Tue Sep 22 1998 - 14:09:01 EST
http://www.biotech.ufl.edu/~fccl/abx_reac Thanks for everyones continued support. Keith Bahjat Graduate Assistant University of Florida College of Medicine kbahjat@ufl.edu Roger A Burger wrote: > I thought there might be some on this list who might find this a useful reference > > Vet Immunol Immunopathol 1998 Jun 30;64(1):1-13 > > > > Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark. > > leucocyte antigen reactive mAbs. > > Roger A. Burger , PThe authors were kind enough to provide us with a pre-press copy of this article, and we have added most of the antibodies in this publication to the database: We have started adding many of the commercially available antibodies which were derived against these less typical species, so we have one resource to look for these reagents. > Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species. > Brodersen R, Bijlsma F, Gori K, Jensen KT, Chen W, Dominguez J, Haverson K, Moore PF, Saalmuller A, Sachs D, Slierendrecht WJ, Stokes C, Vainio O, Zuckermann F, Aasted B > 213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions on the following markers: CD1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43, 44, 45, 45R, 46, 49, 61, 62L, TCR gamma/delta, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new hD > Research Assistant Professor, Immunology > Utah State University > Logan, UT 84322-6895 > Voice: 801-797-2042 > FAX: 801-797-4054 > E-mail: Roger@cpd2.usu.edu
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