Re: M7

From: Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Date: Tue Sep 22 1998 - 06:47:25 EST

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We use 41+61 in FITC and 42b in PE with 45PerCP. We get alot of secondary
leukemias and are always thinking of the possibility of megakaryocytic
lineage.

	Maryalice

>Dear All,
>
>I'd like to get a concensus opinion from the clinical "flowers" since I'm
>having a "difference of opinion" with a flow lab here.
>Which CD do you use for detecting M7's - CD41, 42b or 61?
>My experience of CD42b is that it is the least reliable of the three and
>can be negative where 41 &/or 61 are positive.
>The lab here (not where I am!) uses 42b on it's own and I'm a bit concerned
>that they're likely to miss some.
>
>Wal Sharp

Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH

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