From: Joe Trotter (trotter@scripps.edu)
Date: Thu Sep 03 1998 - 14:40:01 EST
Hello to all, We have been using Criterion azide free isotonic saline as a sheath buffer for several years in both the sorters and analyzers. Recently, the operation was bought out and the buffer is now manufactured at a new site. The latest lot seems toxic to Jurkat cells, yet appears OK with a few other sorts we have done with other cell types. Their QC person informed me they do not screen the water for endotoxins... so, anyone else had any problems with any cells exposed to recent lots of Criterion diluent 2? We found that Jurkat cells lysed overnight when exposed to the buffer for a period of time. I'm hoping this is an anomaly. Joe Joe Trotter Director, Flow Cytometry Mailstop Imm-20 The Scripps Research Institute 10666 North Torrey Pines Rd. La Jolla, California 92037
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