Optimal sheath fluid for cell sorting

From: Simon_Q_Rice@sbphrd.com
Date: Thu Aug 06 1998 - 03:14:23 EST 

To: cyto-inbox
cc:
From:     Simon Q Rice @ SB_PHARM_RD
Date:     06-Aug-98 09:14:23 AM
Subject:  Optimal sheath fluid for cell sorting
Categories:

Dear Flowers,
A question for the cell sorters amongst you.....

I am sorting cultured cells into 96-well plates (wells containing
appropriate culture medium) and am currently using PBS as a sheath fluid.
Experience tells me that cultured cells aren't too keen on PBS for long
periods of time and I wondered if this could have a deleterious effect on
the cells.  Therefore, what alternative sheath fluid, if any, would you
recommend?

Apologies if this is a naive question.

Thank you in anticipation

Simon





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