Re: FACS

From: PETER BERCZ (BERCZ.PETER@EPAMAIL.EPA.GOV)
Date: Wed Aug 05 1998 - 13:10:45 EST


** Reply Requested When Convenient **

There are FACS's (that is  fluorescence activated cell sortes) which
are definitely  NOT based on the flow principle, and example is a
MERIDIAN ULTIMA confocal cell sorter. This  device indeed sorts  cells
based on  their laser activated fluorescence, while it has nothing to do
with  flow cytometry at all. I agree "FACS"  must not be used in 
publications. (Besides I have a distinct aversion  to acronyms. Why ?
you may ask:  Please consider ,  the term "FISH"  has been 100 %
expropriated by the "fluorescent in situ hybridizing" community, saf 
fish, pronto you are a hybridizer. Give me a break,  what am I to do
when I work with fish (piscine) cells, like  lymphocytes from carp, 
brown bullheads and stonrollers? When the  time comes, and we have 
fluorescence labelled gene probes for  stone rollers, as it may happen
soon,  am I going to report FISH in fish? acronym


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