From: Johanna Vanadrichem (Johanna.Vanadrichem@epfl.ch)
Date: Wed Aug 05 1998 - 02:31:35 EST
Hello flowers, Now we finally have our own flowcytometer, I want to do some experiments with E.coli. The first runs I did were with a normal E.coli versus an E.coli producing GFP. Wenn I measure both seperately I get good signals on the fsc and the ssc, as well as for the GFP signal. However if I mix both populations I don't get two peaks, but a decrease of the GFP signal. Furthermore if I measure a non GFP culture after a GFP culture I still find GFP producing cells. Washing with triton X100 and subsequent flushing with water helps a bit but not effective enough. Hope someone can give me some advise how to solve these probléems. Thanks, Johanna -------------------------------------------------------------------- Prénom Nom: Dr. Johanna H. M. van Adrichem Institut: DC-IGC-LBTC Ecole Polytechnique Fédérale de Lausanne (EPFL): CH-1015 Lausanne, Suisse tél: (+41 21) 693 6135 NEW email: Johanna.Vanadrichem@epfl.ch ------------------------- Eudora Pro 3.0 -----------------------------
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