From: Joe Trotter (trotter@scripps.edu)
Date: Tue Jul 28 1998 - 14:30:58 EST
Eric, I'm not sure if you have the TSO, and therefore unsure if your sample is setup with sheath bias or not (as is the default TSO upgrade). In any event, the older regulators with bias and the new can be rebuilt - but it is a pain and you'll need a kit. I think you'll need the obvious - a scheme to dry the air. We have hydrophobic filters in a couple of key places between input and sensitive regulators, and in the sample air line to keep any mistakes from allowing fluid to sneak back up the sample air line. The filters are the same ones as in the FACScan PM kits - Acrodisc CR PTFE 1.0 um from Gelman. Joe On Mon, 27 Jul 1998, Eric Van Buren wrote: > > Last Friday we had the sample differential on our FACS Vantage fail. It > appears to have "frozen up" due to some mineral deposits. Has anyone tried > to clean one of these? Is it practical? > > The source of the deposits appears to be our pressure pump. On humid days, > it blows moist air through the cytometer. Has anyone else dealt with this > sort of problem? I'm interested in hearing remedies before I "re-invent the > wheel". > > Thanks, > Eric > > /\/\/\_ Eric Van Buren, aa9080@wayne.edu > \ \ \ Karmanos Cancer Institute and Immunology & Microbiology > \_^_/ Wayne State University, Detroit, Michigan, USA > > > Joe Trotter Director, Flow Cytometry Mailstop Imm-20 The Scripps Research Institute 10666 North Torrey Pines Rd. La Jolla, California 92037
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